| * | M | 0.0 |
| # | C | 0.0 |
| @ | S | 0.0 |
| true | Use criteria |
| 1.8 | Minimum +1 XCorr |
| 2.5 | Minimum +2 XCorr |
| 3.5 | Minimum +3 XCorr |
| 0.08 | Minimum DeltCN |
| 1 | Minimum charge state |
| 3 | Maximum charge state |
| 0.0 | Minimum ion proportion |
| 1000 | Maximum Sp rank |
| -1.0 | Minimum Sp score |
| Include | Modified peptide inclusion |
| Any | Tryptic status requirement |
| true | Multiple, ambiguous IDs allowed |
| Ignore | Peptide validation handling |
| XCorr | Purge duplicate peptides by protein |
| false | Include only loci with unique peptide |
| false | Remove subset proteins |
| Ignore | Locus validation handling |
| 0 | Minimum modified peptides per locus |
| 10 | Minimum redundancy for low coverage loci |
| 2 | Minimum peptides per locus |
| Validation Status | Locus | Sequence Count | Spectrum Count | Sequence Coverage | Length | MolWt | pI | Descriptive Name |
| Locus | # of identical peptides | # of differing peptides |
| U | LeptoII_scaff_2_GENE_114 | 39 | 174 | 91.5% | 153 | 17309 | 7.3 | # Gene_name: AhpC # Function: Peroxiredoxin # e-28 # pI:6.82 MW:17309 |
| U | LeptoII_scaff_137_GENE_6 | 33 | 646 | 91.4% | 128 | 13303 | 5.2 | # Gene_name: RplL # Function: Ribosomal protein L7/L12 # e-20 # pI:5.14 MW:13303 |
| U | LeptoII_scaff_317_GENE_7 | 26 | 150 | 89.7% | 136 | 15155 | 5.5 | # Gene_name: - # Function: Hypothetical protein # pI:5.34 MW:15155 |
| U | LeptoII_scaff_140_GENE_1 | 11 | 368 | 89.7% | 68 | 7359 | 6.5 | # Gene_name: CspC # Function: Cold shock proteins # e-19 # pI:6.04 MW:7359 |
| U | Unass_bact_scaff_1107_GENE_1 | 8 | 14 | 86.0% | 57 | 6364 | 6.5 | # Gene_name: InfC # Function: Translation initiation factor IF3 # e-12 # pI:6.08 MW:6364 |
| Filename | XCorr | DeltCN | ObsM+H+ | CalcM+H+ | SpR | SpScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Biofilm_Sample_B_2D_MP_1stmz_Run1_11.2132.2132.3 | 3.8216 | 0.5936 | 2006.5745 | 2006.3525 | 1 | 556.0 | 40.3% | 1 | -.VIGPEGEQLGIFPTHVAIK.K | 33 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_10.1917.1917.2 | 4.955 | 0.6219 | 2007.0522 | 2006.3525 | 1 | 1144.4 | 58.3% | 2 | -.VIGPEGEQLGIFPTHVAIK.K | 22 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_08.1519.1519.2 | 5.566 | 0.5901 | 2373.9722 | 2373.7266 | 1 | 1462.2 | 57.1% | 2 | K.KAEEQGFDLVEVSPTAKPPVCK.M | 22 | |
| Biofilm_Sample_B_2D_MP_1stmz_Run1_09.1724.1724.3 | 5.2028 | 0.5887 | 2374.1943 | 2373.7266 | 1 | 1254.1 | 38.1% | 1 | K.KAEEQGFDLVEVSPTAKPPVCK.M | 33 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_06.1602.1602.2 | 4.8883 | 0.5663 | 2244.912 | 2245.5525 | 1 | 1218.8 | 52.5% | 2 | K.AEEQGFDLVEVSPTAKPPVCK.M | 22 | |
| Biofilm_Sample_B_2D_MP_1stmz_Run1_07.1785.1785.3 | 5.6122 | 0.5473 | 2245.7644 | 2245.5525 | 1 | 1803.4 | 47.5% | 3 | K.AEEQGFDLVEVSPTAKPPVCK.M | 33 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_04.0799.0799.1 | 2.3433 | 0.3842 | 948.54 | 949.0662 | 1 | 422.8 | 75.0% | 1 | K.YMYEQSK.K | 11 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_08.0708.0708.1 | 2.6229 | 0.3418 | 1078.57 | 1077.2404 | 1 | 492.8 | 71.4% | 2 | K.YMYEQSKK.V | 11 |
| U | LeptoII_scaff_21_GENE_23 | 19 | 89 | 84.2% | 139 | 15326 | 5.4 | # Gene_name: - # Function: Hypothetical protein # pI:5.26 MW:15326 |
| U | LeptoII_scaff_48_GENE_19 | 27 | 232 | 81.5% | 232 | 25566 | 9.1 | # Gene_name: PorG # Function: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, gamma subunit # e-71 # pI:9.11 MW:25566 |
| U | LeptoII_scaff_59_GENE_9 | 16 | 62 | 80.0% | 80 | 8760 | 7.2 | # Gene_name: SirA # Function: Predicted redox protein, regulator of disulfide bond formation # e-18 # pI:6.56 MW:8760 |
| U | Unass_bact_scaff_753_GENE_5 | 4 | 6 | 79.5% | 73 | 8132 | 8.5 | - # Function: Hypothetical protein # pI:8.08 MW:8132 |
| Filename | XCorr | DeltCN | ObsM+H+ | CalcM+H+ | SpR | SpScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_04.1304.1304.1 | 2.4407 | 0.3452 | 979.64 | 980.1491 | 1 | 590.9 | 71.4% | 1 | R.SLIYLNEK.T | 11 | |
| Biofilm_Sample_B_2D_MP_2ndmz_Run1_04.2065.2065.2 | 5.2506 | 0.5903 | 1872.7322 | 1873.1136 | 1 | 1746.7 | 67.6% | 2 | K.TITSLALEAGIDPTNLSR.F | 22 | |
| Biofilm_Sample_B_2D_MP_3rdmz_Run1_11.0844.0844.1 | 2.4419 | 0.4469 | 1347.72 | 1348.4985 | 1 | 593.2 | 59.1% | 2 | R.FLKGHSTVSDEK.M | 11 | |
| Biofilm_Sample_B_2D_MP_1stmz_Run1_12.2204.2204.3 | 5.0319 | 0.5664 | 2194.0745 | 2193.5945 | 1 | 2052.8 | 48.7% | 1 | K.VLEHLGVDPVTGTLKPVVYR.W | 33 |
| U | LeptoII_scaff_30_GENE_30 | 17 | 101 | 78.5% | 107 | 12389 | 5.3 | - # Function: Hypothetical protein # pI:5.29 MW:12389 |
| U | LeptoII_scaff_6_GENE_22 | 24 | 263 | 76.7% | 116 | 13158 | 9.0 | - # Function: Hypothetical protein # pI:9.01 MW:13158 |
| U | LeptoII_scaff_27_GENE_40 | 45 | 107 | 73.8% | 225 | 25652 | 5.3 | # Gene_name: PspA # Function: Phage shock protein A (IM30), suppresses sigma54-dependent transcription # e-12 # pI:5.16 MW:25652 |
| U | LeptoII_scaff_120_GENE_7 | 17 | 52 | 73.2% | 138 | 15726 | 7.5 | # Gene_name: Ndk # Function: Nucleoside diphosphate kinase # e-41 # pI:6.91 MW:15726 |
| U | LeptoII_scaff_32_GENE_38 | 18 | 157 | 73.2% | 112 | 12260 | 9.0 | # Gene_name: GlnK # Function: Nitrogen regulatory protein PII # e-31 # pI:8.96 MW:12260 |
| U | LeptoII_scaff_374_GENE_2 | 15 | 25 | 72.3% | 231 | 25922 | 6.1 | - # Function: Hypothetical protein # pI:5.76 MW:25922 |
| U | LeptoII_scaff_59_GENE_20 | 14 | 67 | 72.1% | 104 | 11088 | 5.0 | # Gene_name: - # Function: Hypothetical protein # pI:4.92 MW:11088 |
| U | LeptoII_scaff_48_GENE_14 | 11 | 36 | 71.8% | 117 | 12964 | 8.4 | # Gene_name: PorB # Function: Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, beta subunit # e-25 # pI:8.54 MW:12964 |
| U | LeptoII_scaff_88_GENE_20 | 2 | 5 | 70.6% | 34 | 3865 | 10.2 | - # Function: Hypothetical protein # pI:10.18 MW:3865 |
| Filename | XCorr | DeltCN | ObsM+H+ | CalcM+H+ | SpR | SpScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Biofilm_Sample_B_2D_MP_3rdmz_Run1_12.3166.3166.1 | 3.6329 | 0.6035 | 1515.13 | 1513.8816 | 1 | 855.5 | 65.4% | 2 | K.VIHFASPSILGMIK.K | 1 |
| * | Biofilm_Sample_B_2D_MP_3rdmz_Run1_11.0914.0914.1 | 2.9461 | 0.4537 | 1209.77 | 1210.3936 | 1 | 479.6 | 72.2% | 3 | K.TQHMISVEHK.- | 1 |
| U | LeptoII_scaff_137_GENE_4 | 23 | 125 | 69.8% | 232 | 25198 | 9.5 | # Gene_name: RplA # Function: Ribosomal protein L1 # e-63 # pI:9.45 MW:25199 |
| U | LeptoII_scaff_141_GENE_1 | 15 | 74 | 69.5% | 95 | 10759 | 8.3 | - # Function: Hypothetical protein # pI:7.90 MW:10759 |
| U | LeptoII_scaff_8_GENE_70 | 15 | 74 | 69.5% | 95 | 10759 | 8.3 | - # Function: Hypothetical protein # pI:7.90 MW:10759 |
| U | LeptoII_scaff_2_GENE_52 | 22 | 39 | 68.9% | 344 | 36903 | 6.2 | # Gene_name: PdxA # Function: Pyridoxal phosphate biosynthesis protein # e-57 # pI:5.83 MW:36903 |
| U | LeptoII_scaff_2_GENE_74 | 14 | 134 | 68.0% | 103 | 11831 | 4.9 | - # Function: Hypothetical protein # pI:4.82 MW:11831 |
| U | LeptoII_scaff_141_GENE_3 | 46 | 370 | 67.9% | 265 | 29387 | 9.7 | # Gene_name: OmpA # Function: Outer membrane protein and related peptidoglycan-associated (lipo)proteins # e-48 # pI:9.74 MW:29387 |
| U | LeptoII_scaff_174_GENE_16 | 16 | 151 | 67.6% | 102 | 10677 | 10.7 | # Gene_name: HimA # Function: Bacterial nucleoid DNA-binding protein # e-14 # pI:10.72 MW:10677 |
| U | LeptoII_scaff_13_GENE_67 | 28 | 131 | 67.2% | 198 | 22071 | 6.6 | # Gene_name: Tsf # Function: Translation elongation factor Ts # e-50 # pI:6.27 MW:22071 |
| U | LeptoII_scaff_120_GENE_14 | 22 | 108 | 66.5% | 176 | 19724 | 8.7 | # Gene_name: CcoO # Function: Cbb3-type cytochrome oxidase, cytochrome c subunit # e-14 # pI:8.85 MW:19724 |
| U | LeptoII_scaff_25_GENE_2 | 22 | 108 | 66.5% | 176 | 19724 | 8.7 | # Gene_name: CcoO # Function: Cbb3-type cytochrome oxidase, cytochrome c subunit # e-14 # pI:8.85 MW:19724 |
| U | Unass_bact_scaff_796_GENE_3 | 15 | 81 | 66.4% | 128 | 13085 | 5.2 | # Gene_name: RplL # Function: Ribosomal protein L7/L12 # e-19 # pI:5.11 MW:13085 |
| U | LeptoII_scaff_222_GENE_9 | 23 | 136 | 66.4% | 110 | 12185 | 5.6 | # Gene_name: TrxA # Function: Thiol-disulfide isomerase and thioredoxins # e-26 # pI:5.42 MW:12185 |
| U | LeptoII_scaff_62_GENE_10 | 32 | 2390 | 66.3% | 374 | 38670 | 7.3 | - # Function: Hypothetical protein # pI:6.71 MW:38671 |
| U | LeptoII_scaff_26_GENE_44 | 14 | 58 | 65.8% | 193 | 21354 | 8.6 | - # Function: Hypothetical protein # pI:8.84 MW:21354 |
| U | LeptoII_scaff_185_GENE_13 | 13 | 28 | 65.8% | 155 | 18224 | 6.6 | - # Function: Hypothetical protein # pI:6.19 MW:18224 |
| U | LeptoII_scaff_13_GENE_68 | 23 | 154 | 65.3% | 259 | 29319 | 7.6 | # Gene_name: RpsB # Function: Ribosomal protein S2 # e-74 # pI:7.07 MW:29319 |
| U | LeptoII_scaff_1108_GENE_1 | 14 | 23 | 65.0% | 160 | 17792 | 6.2 | # Gene_name: GreA # Function: Transcription elongation factor # e-36 # pI:5.83 MW:17792 |
| U | LeptoII_scaff_25_GENE_60 | 14 | 23 | 65.0% | 160 | 17792 | 6.2 | # Gene_name: GreA # Function: Transcription elongation factor # e-36 # pI:5.83 MW:17792 |
| U | LeptoII_scaff_22_GENE_45 | 25 | 78 | 64.8% | 182 | 20762 | 5.6 | # Gene_name: Ppa # Function: Inorganic pyrophosphatase # e-40 # pI:5.42 MW:20762 |
| U | LeptoII_scaff_21_GENE_11 | 15 | 34 | 64.4% | 87 | 9750 | 11.5 | - # Function: Hypothetical protein # pI:11.49 MW:9751 |
| Filename | XCorr | DeltCN | ObsM+H+ | CalcM+H+ | SpR | SpScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Biofilm_Sample_B_2D_MP_2ndmz_Run1_11.0872.0872.1 | 1.9016 | 0.1494 | 916.8 | 917.1368 | 4 | 331.2 | 71.4% | 1 | K.ATLKDIKK.N | 1 |
| * | Biofilm_Sample_B_2D_MP_1stmz_Run1_08.1137.1137.1 | 2.0436 | 0.1916 | 804.5 | 804.983 | 18 | 63.7 | 66.7% | 1 | R.QAVSMIR.T | 1 |
| * | Biofilm_Sample_B_2D_MP_1stmz_Run1_09.1048.1048.2 | 2.6612 | 0.2434 | 1089.8121 | 1089.2773 | 1 | 791.9 | 77.8% | 1 | K.KVETLVSQGK.K | 2 |
| * | Biofilm_Sample_B_2D_MP_2ndmz_Run1_08.0893.0893.1 | 2.9138 | 0.346 | 1090.77 | 1089.2773 | 1 | 486.6 | 66.7% | 2 | K.KVETLVSQGK.K | 1 |
| * | Biofilm_Sample_B_2D_MP_3rdmz_Run1_11.0854.0854.1 | 3.2259 | 0.486 | 1216.9 | 1217.4514 | 1 | 625.6 | 75.0% | 15 | K.KVETLVSQGKK.E | 1 |
| * | Biofilm_Sample_B_2D_MP_1stmz_Run1_12.1889.1889.2 | 3.5409 | 0.3202 | 1217.5322 | 1217.4514 | 1 | 1405.8 | 85.0% | 1 | K.KVETLVSQGKK.E | 2 |
| * | Biofilm_Sample_B_2D_MP_2ndmz_Run1_12.3103.3103.3 | 5.813 | 0.6124 | 3315.1143 | 3315.8381 | 1 | 1172.3 | 30.8% | 1 | K.KVETLVSQGKKEEAVVALGEVVPVIDHAVNR.R | 3 |
| * | Biofilm_Sample_B_2D_MP_2ndmz_Run1_07.0833.0833.1 | 2.7273 | 0.3912 | 1088.58 | 1089.2773 | 1 | 623.9 | 77.8% | 1 |